Anti-GAD65 兔多克隆抗体-神经递质,神经元-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-GAD65 兔多克隆抗体

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￥1900

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货号： K134686-100

预测分子量： 65 kDa

观测分子量： 65 kDa

别名： GAD2,GAD65,Glutamate decarboxylase 2,65 kDa glutamic acid decarboxylase,DCE 2,Glutamic Acid Decarboxylase 2,Glutamic Acid Decarboxylase 65

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	谷氨酸脱羧酶2
别名	GAD2,GAD65,Glutamate decarboxylase 2,65 kDa glutamic acid decarboxylase,DCE 2,Glutamic Acid Decarboxylase 2,Glutamic Acid Decarboxylase 65
Uniprot ID	P48320
免疫原	小鼠GAD65重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	65 kDa / 65 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:海马体, 脑

背景

Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. In mammals, GAD exists in two isoforms with molecular weights of 67 and 65 kDa (GAD67 and GAD65). GAD67 and GAD65 are expressed in the brain where GABA is used as a neurotransmitter, and they are also expressed in the insulin-producing β-cells of the pancreas. GAD65, synthesizes GABA for neurotransmission, and therefore is only necessary at nerve terminals and synapses. In order to aid in neurotransmission, GAD65 forms a complex with Heat Shock Cognate 70 (HSC70), cysteine string protein (CSP) and Vesicular GABA transporter VGAT, which, as a complex, helps package GABA into vesicles for release during neurotransmission.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测GAD65蛋白(货号 K134686).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.