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Anti-TNF Receptor II 兔多克隆抗体
¥1900
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货号: K1333039-100
预测分子量: 48 kDa
观测分子量: 73 kDa
别名: Tumor necrosis factor receptor 2,TNFR2,p75,Tumor necrosis factor receptor type II,TNFRII,p80 TNF-alpha receptor,CD120b,Etanercept,TBP-2,TBPII,TNFRSF1B,TNFBR
应用 | 种属
WB H
规格:
产品信息
蛋白质全称 肿瘤坏死因子受体超家族成员1B
别名 Tumor necrosis factor receptor 2,TNFR2,p75,Tumor necrosis factor receptor type II,TNFRII,p80 TNF-alpha receptor,CD120b,Etanercept,TBP-2,TBPII,TNFRSF1B,TNFBR
Uniprot ID P20333
免疫原 KLH偶联的人TNF Receptor II合成肽
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 48 kDa / 73 kDa
应用
WB 免疫印迹
物种:人
稀释比:1:500-1:1000
阳性样品:Jurkat细胞
背景
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测TNF Receptor II蛋白(货号 K1333039).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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