Anti-AP-2 complex subunit alpha-1 兔多克隆抗体-细胞骨架-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-AP-2 complex subunit alpha-1 兔多克隆抗体

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货号： K1333348-100

预测分子量： 108 kDa

观测分子量： 108 kDa

别名： 100 kDa coated vesicle protein A,Adaptor protein complex AP-2 subunit alpha-1,Adaptor-related protein complex 2 subunit alpha-1,Alpha-adaptin A,Alpha1-adaptin,Ap2a1,Adtaa,Clapa1

应用 | 种属

WB H

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	AP-2复合体α-1亚基
别名	100 kDa coated vesicle protein A,Adaptor protein complex AP-2 subunit alpha-1,Adaptor-related protein complex 2 subunit alpha-1,Alpha-adaptin A,Alpha1-adaptin,Ap2a1,Adtaa,Clapa1
Uniprot ID	P17426
免疫原	小鼠Ap2a1重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	108 kDa / 108 kDa

应用

WB 免疫印迹

物种:人

稀释比:1:500-1:1000

阳性样品:A-431细胞

背景

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测AP-2 complex subunit alpha-1蛋白(货号 K1333348).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.