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Anti-HES5 兔多克隆抗体
¥1900
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货号: K1334194-100
预测分子量: 18 kDa
观测分子量: 18 kDa
别名: Hairy and enhancer of split 5,Hes5,bHLHb38,Class B basic helix-loop-helix protein 38,Hairy and enhancer of split 5,hes family bHLH transcription factor 5
应用 | 种属
WB M,R
规格:
产品信息
蛋白质全称 转录因子HES-5
别名 Hairy and enhancer of split 5,Hes5,bHLHb38,Class B basic helix-loop-helix protein 38,Hairy and enhancer of split 5,hes family bHLH transcription factor 5
Uniprot ID P70120
免疫原 小鼠HES5重组蛋白
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 18 kDa / 18 kDa
应用
WB 免疫印迹
物种:小鼠,大鼠
稀释比:1:500-1:1000
阳性样品:海马区
背景
Hairy and Enhancer-of-split homologues 5 (HES5) is a helix-loop-helix transcriptional factor that negatively regulates cell differentiation during embryogenesis. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. HES5 as a transcriptional repressor of genes that require a bHLH protein for their transcription and plays an important role as neurogenesis negative regulator. HES5 is reported to be a down stream effector of the Notch Signaling Pathway and is expressed in advanced ovarian serous adenocarcinomas as well as in cervical squamous carcinoma.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测HES5蛋白(货号 K1334194).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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