Anti-ERp57 兔多克隆抗体-分子伴侣-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-ERp57 兔多克隆抗体

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货号： K1340227-100

预测分子量： 57 kDa

观测分子量： 57 kDa

别名： 58 kDa microsomal protein,Disulfide isomerase ER 60,ER protein 57,ER protein 60,ER60,ERp57,ERp60,ERp61,GRP57,GRP58,HsT17083,P58,PDIA3,PI PLC,Protein disulfide isomerase A3

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	蛋白质二硫键异构酶A3
别名	58 kDa microsomal protein,Disulfide isomerase ER 60,ER protein 57,ER protein 60,ER60,ERp57,ERp60,ERp61,GRP57,GRP58,HsT17083,P58,PDIA3,PI PLC,Protein disulfide isomerase A3
Uniprot ID	P27773
免疫原	小鼠ERp57重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	57 kDa / 57 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:1000-1:2000

阳性样品:脑, 肾

背景

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测ERp57蛋白(货号 K1340227).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.