Anti-Huntingtin Associated Protein 1 兔多克隆抗体-神经退行性疾病-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-Huntingtin Associated Protein 1 兔多克隆抗体

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￥1900

加入对比

货号： K1343218-100

预测分子量： 76 kDa

观测分子量： 76-80 kDa

亚细胞定位：细胞质, 细胞连接

别名： HAP-1,HAP1,HAP2,hHLP1,HIP5,HLP,HLP1,Neuroan 1

应用 | 种属

WB M,R

IHC/IF M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	Huntingtin蛋白关联蛋白1
别名	HAP-1,HAP1,HAP2,hHLP1,HIP5,HLP,HLP1,Neuroan 1
Uniprot ID	O35668
免疫原	小鼠HAP1重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞质, 细胞连接
预测分子量./观测分子量.	76 kDa / 76-80 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:睾丸

IHC/IF 免疫组织化学/免疫荧光

物种:小鼠,大鼠

稀释比:1:1000-1:2000

阳性样品:—

背景

Originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT possibly affecting HAP1 interaction properties. Both HTT and HAP1 are involved in intracellular trafficking and HAP1 is proposed to link HTT to motor proteins and/or transport cargos. Seems to play a role in vesicular transport within neurons and axons such as from early endosomes to late endocytic compartments and to promote neurite outgrowth. The vesicular transport function via association with microtubule-dependent transporters can be attenuated by association with mutant HTT. Involved in the axonal transport of BDNF and its activity-dependent secretion; the function seems to involve HTT, DCTN1 and a complex with SORT1.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Huntingtin Associated Protein 1蛋白(货号 K1343218).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.