Anti-MRF 兔多克隆抗体-转录因子-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-MRF 兔多克隆抗体

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￥1900

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货号： K1343278-100

预测分子量： 123 kDa

观测分子量： 120 kDa

别名： C11orf9,MRF,Ndt80,pqn-47

应用 | 种属

WB H

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	髓鞘调节因子[剪切形式]
别名	C11orf9,MRF,Ndt80,pqn-47
Uniprot ID	Q3UR85
免疫原	小鼠MYRF重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	123 kDa / 120 kDa

应用

WB 免疫印迹

物种:人

稀释比:1:300-1:500

阳性样品:HeLa细胞, Hep G2细胞

背景

[Myelin regulatory factor]: Constitutes a precursor of the transcription factor. Mediates the autocatalytic cleavage that releases the Myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes.; [Myelin regulatory factor, C-terminal]: Membrane-bound part that has no transcription factor activity and remains attached to the endoplasmic reticulum membrane following cleavage.; [Myelin regulatory factor, N-terminal]: Transcription factor that specifically activates expression of myelin genes such as MBP, MOG, MAG, DUSP15 and PLP1 during oligodendrocyte (OL) maturation, thereby playing a central role in oligodendrocyte maturation and CNS myelination . Specifically recognizes and binds DNA sequence 5'-CTGGYAC-3' in the regulatory regions of myelin-specific genes and directly activates their expression. Not only required during oligodendrocyte differentiation but is also required on an ongoing basis for the maintenance of expression of myelin genes and for the maintenance of a mature, viable oligodendrocyte phenotype.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测MRF蛋白(货号 K1343278).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 300稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.