Anti-TOP1MT 兔多克隆抗体-DNA合成-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-TOP1MT 兔多克隆抗体

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￥1900

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货号： K1345759-100

预测分子量： 69 kDa

观测分子量： 66 kDa

别名： Topoisomerase I mitochondrial,2900052H09Rik

应用 | 种属

WB R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	线粒体DNA拓扑异构酶I
别名	Topoisomerase I mitochondrial,2900052H09Rik
Uniprot ID	Q8R4U6
免疫原	KLH偶联的小鼠TOP1MT合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	69 kDa / 66 kDa

应用

WB 免疫印迹

物种:大鼠

稀释比:1:2000-1:3000

阳性样品:结肠, 肺

背景

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测TOP1MT蛋白(货号 K1345759).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 2000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.