Anti-LONP1/Lon 兔多克隆抗体-缺氧-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-LONP1/Lon 兔多克隆抗体

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￥1900

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货号： K1334440-100

预测分子量： 106 kDa

观测分子量： 106 kDa

别名： Lon protease-like protein,LONP,Mitochondrial ATP-dependent protease Lon,Serine protease 15,Lonp1,Prss15

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	线粒体Lon蛋白酶同源物
别名	Lon protease-like protein,LONP,Mitochondrial ATP-dependent protease Lon,Serine protease 15,Lonp1,Prss15
Uniprot ID	Q8CGK3
免疫原	小鼠LONP1重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	106 kDa / 106 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:心

背景

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix. LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover. It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测LONP1/Lon蛋白(货号 K1334440).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.