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Anti-c-Jun 兔多克隆抗体
¥1900
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货号: K1334812-100
预测分子量: 36 kDa
观测分子量: 43 kDa
别名: Activator protein 1,AP1,Proto-oncogene c-Jun,V-jun avian sarcoma virus 17 oncogene homolog,p39,JUN,Transcription factor AP-1,Proto oncogene cJun,AP-1 transcription factor subunit
应用 | 种属
WB H,M,R
规格:
产品信息
蛋白质全称 转录因子AP-1
别名 Activator protein 1,AP1,Proto-oncogene c-Jun,V-jun avian sarcoma virus 17 oncogene homolog,p39,JUN,Transcription factor AP-1,Proto oncogene cJun,AP-1 transcription factor subunit
Uniprot ID P05412
免疫原 KLH偶联的人c-Jun合成肽
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 36 kDa / 43 kDa
应用
WB 免疫印迹
物种:人,小鼠,大鼠
稀释比:1:500-1:1000
阳性样品:HeLa细胞, Hep G2细胞
背景
c-Jun is a protein that in humans is encoded by the JUN gene. c-Jun in combination with c-Fos, forms the AP-1early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the c-jun gene. It is activated through double phosphorylation by the JNK pathway but has also a phosphorylation-independent function. c-jun knockout is lethal, but transgenic animalswith a mutated c-jun that cannot be phosphorylated (termed c-junAA) can survive.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测c-Jun蛋白(货号 K1334812).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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