Anti-PCB 兔多克隆抗体-能量代谢-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-PCB 兔多克隆抗体

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￥1900

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货号： K1339006-100

预测分子量： 130 kDa

观测分子量： 130 kDa

别名： Pyruvic carboxylase,PCB,Pc,Pcx,pyruvate Carboxylase

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	丙酮酸羧化酶（线粒体）
别名	Pyruvic carboxylase,PCB,Pc,Pcx,pyruvate Carboxylase
Uniprot ID	Q05920
免疫原	KLH偶联的小鼠PCB合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	130 kDa / 130 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:1000-1:2000

阳性样品:海马区

背景

PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测PCB蛋白(货号 K1339006).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.