Anti-GAA1 兔多克隆抗体-细胞器标志物-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-GAA1 兔多克隆抗体

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货号： K1340776-100

亚细胞定位：内质网

别名： GAA1,GAA1 protein homolog,GPAA1,hGAA1,GPI anchor attachment protein 1,mGAA1,Anchor attachment protein 1 (Gaa1p,yeast) homolog,GPI transamidase subunit

应用 | 种属

IHC/IF R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	糖基磷脂酰肌醇锚定附着1蛋白
别名	GAA1,GAA1 protein homolog,GPAA1,hGAA1,GPI anchor attachment protein 1,mGAA1,Anchor attachment protein 1 (Gaa1p,yeast) homolog,GPI transamidase subunit
Uniprot ID	Q9WTK3
免疫原	小鼠GAA1重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	内质网

应用

IHC/IF 免疫组织化学/免疫荧光

物种:大鼠

稀释比:1:2000-1:4000

阳性样品:肾

背景

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif. It has 2 isoforms produced by alternative splicing and the full length protein has 2 glycosylation sites. This antibody is specific to GPAA1.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测GAA1蛋白(货号 K1340776).
样品: 大鼠肾, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (KSG1201), 98℃, 20分钟.
—抗: 1: 3000稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.