Anti-PAM/Peptidyl-glycine alpha-amidating monooxygenase 兔多克隆抗体-氨基酸代谢-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-PAM/Peptidyl-glycine alpha-amidating monooxygenase 兔多克隆抗体

咨询

￥1900

加入对比

货号： K1346077-100

亚细胞定位：细胞质

别名： PAL,PAM,Peptidylamidoglycolate lyase,PHM

应用 | 种属

IHC/IF M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

在线预览下载说明书

产品信息

蛋白质全称	肽基甘氨酸α-酰胺化单加氧酶
别名	PAL,PAM,Peptidylamidoglycolate lyase,PHM
Uniprot ID	P97467
免疫原	小鼠PAM/Peptidyl-glycine alpha-amidating monooxygenase重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞质

应用

IHC/IF 免疫组织化学/免疫荧光

物种:小鼠,大鼠

稀释比:1:700-1:1400

阳性样品:心

背景

Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate. The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate. Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (Probable).

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测PAM/Peptidyl-glycine alpha-amidating monooxygenase蛋白(货号 K1346077).
样品: 小鼠心, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (KSG1203), 98℃, 20分钟.
—抗: 1: 1100稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.

IHC检测PAM/Peptidyl-glycine alpha-amidating monooxygenase蛋白(货号 K1346077).
样品: 大鼠心, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (KSG1203), 98℃, 20分钟.
—抗: 1: 1100稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.