Anti-Podoplanin/gp36 兔多克隆抗体-细胞连接-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-Podoplanin/gp36 兔多克隆抗体

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￥1900

加入对比

货号： K1346329-100

预测分子量： 18 kDa

观测分子量： 38 kDa

别名： AGGRUS,D2 40,D2 40,M2A,Glycoprotein 36,GP36,Gp38,GP40,HT1A 1,M2A,OTS8,PA2.26,PA2.26 antigen,PDPN,podoplanin,PSEC0025,T1 alpha,T1A,T1A 2

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	足细胞膜蛋白
别名	AGGRUS,D2 40,D2 40,M2A,Glycoprotein 36,GP36,Gp38,GP40,HT1A 1,M2A,OTS8,PA2.26,PA2.26 antigen,PDPN,podoplanin,PSEC0025,T1 alpha,T1A,T1A 2
Uniprot ID	Q62011
免疫原	KLH偶联的小鼠Podoplanin合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	18 kDa / 38 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:300-1:800

阳性样品:睾丸

背景

Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation. Interaction with CD9, on the contrary, attenuates platelet aggregation and pulmonary metastasis induced by PDPN. Mediates effects on cell migration and adhesion through its different partners. Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness. Interaction with CD44 promotes directional cell migration in epithelial and tumor cells.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Podoplanin/gp36蛋白(货号 K1346329).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.