Anti-Fibulin 5 兔多克隆抗体-细胞连接-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-Fibulin 5 兔多克隆抗体

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￥1900

加入对比

货号： K1339219-100

预测分子量： 50 kDa

观测分子量： 50 kDa

别名： FIBL-5,Developmental arteries and neural crest EGF-like protein,Dance,Fbln5,Urine p50 protein,UP50,ARMD3,EVEC

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	纤维蛋白
别名	FIBL-5,Developmental arteries and neural crest EGF-like protein,Dance,Fbln5,Urine p50 protein,UP50,ARMD3,EVEC
Uniprot ID	Q9WVH9
免疫原	KLH偶联的小鼠Fibulin 5合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	50 kDa / 50 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:脑

背景

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Fibulin 5蛋白(货号 K1339219).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.