Anti-ERK1 兔多克隆抗体-MAPK通路-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-ERK1 兔多克隆抗体

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￥1900

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货号： K1346404-100

预测分子量： 43 kDa

观测分子量： 43 kDa

别名： MAPK3,ERK-1,ERK1,ERT2,HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,p44-ERK1,p44-MAPK,mitogen-activated protein kinase 3; MAPK1,ERK,ERK-2,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk,p42-MAPK

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	丝裂原活化蛋白激酶3
别名	MAPK3,ERK-1,ERK1,ERT2,HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,p44-ERK1,p44-MAPK,mitogen-activated protein kinase 3; MAPK1,ERK,ERK-2,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk,p42-MAPK
Uniprot ID	Q63844
免疫原	KLH偶联的小鼠ERK1合成磷酸肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	43 kDa / 43 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:300-1:1000

阳性样品:脑, 肺

背景

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X2-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测ERK1蛋白(货号 K1346404).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.