Anti-MEKK2 兔多克隆抗体-MAPK通路-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-MEKK2 兔多克隆抗体

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货号： K135256-100

预测分子量： 70 kDa

观测分子量： 70 kDa

别名： Map3k2,MAP3K2,MEKK2,MEKK2B,Mitogen-activated protein kinase kinase kinase 2

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	丝裂原活化蛋白激酶激酶激酶2
别名	Map3k2,MAP3K2,MEKK2,MEKK2B,Mitogen-activated protein kinase kinase kinase 2
Uniprot ID	Q61083
免疫原	小鼠MEKK2重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	70 kDa / 70 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:胸腺

背景

Mitogen-activated protein kinase kinase kinase 2 is an enzyme encoded by the MAP3K2 gene. The protein is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate IkappaB kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测MEKK2蛋白(货号 K135256).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.