Anti-MEK1 小鼠单克隆抗体-MAPK通路-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-MEK1 小鼠单克隆抗体

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￥1900

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货号： K236915-100

预测分子量： 43 kDa

观测分子量： 45 kDa

别名： MAP2K1,CFC3,MAPKK1,MEK1,MKK1,PRKMK1,mitogen activated protein kinase kinase 1,ERK activator kinase 1,MAP kinase kinase 1

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	双特异性丝裂原活化蛋白激酶激酶1
别名	MAP2K1,CFC3,MAPKK1,MEK1,MKK1,PRKMK1,mitogen activated protein kinase kinase 1,ERK activator kinase 1,MAP kinase kinase 1
Uniprot ID	P31938
免疫原	KLH偶联的小鼠MEK1合成肽
抗体亚型	IgG1, κ
克隆号	7C8E1
纯化方式	亲和纯化
预测分子量./观测分子量.	43 kDa / 45 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:1000-1:2000

阳性样品:肾, 肺

背景

The protein is a member of the dual-specificity protein kinase family that acts as amitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon activation by a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测MEK1蛋白(货号 K236915).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗小鼠IgG (H+L) (货号KB69903), 1: 5000稀释, 室温孵育1小时.