Anti-MK-3 兔多克隆抗体-MAPK通路-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-MK-3 兔多克隆抗体

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￥1900

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货号： K135082-100

预测分子量： 43 kDa

观测分子量： 43 kDa

别名： MAPKAPK3,3PK,MAPKAP-K3,MAPKAP3,MAPKAPK-3,MK-3,mitogen-activated protein kinase-activated protein kinase 3,MDPT3,MAPKAP kinase 3,Chromosome 3p kinase

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	MAP激酶活化蛋白激酶3
别名	MAPKAPK3,3PK,MAPKAP-K3,MAPKAP3,MAPKAPK-3,MK-3,mitogen-activated protein kinase-activated protein kinase 3,MDPT3,MAPKAP kinase 3,Chromosome 3p kinase
Uniprot ID	Q3UMW7
免疫原	小鼠MK-3重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	43 kDa / 43 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:骨骼肌

背景

MAP kinase-activated protein kinase 3 is a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测MK-3蛋白(货号 K135082).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.