Recombinant Anti-MBP Tag antibody (兔单克隆抗体)-标签抗体,重组抗体-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Recombinant Anti-MBP Tag antibody (兔单克隆抗体)

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￥980

加入对比

货号： K5450129-100

预测分子量： 43 kDa

观测分子量： 75 kDa

别名： Maltose binding protein

应用 | 种属

WB Species independent

ICC/IF Species independent

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	麦芽糖结合蛋白标签
别名	Maltose binding protein
免疫原	纯化的Maltose Binding fusion protein在E.coli中表达
抗体亚型	IgG, κ
克隆号	SB345
纯化方式	亲和纯化
预测分子量./观测分子量.	43 kDa / 75 kDa

应用

WB 免疫印迹

物种:Species independent

稀释比:1:1000-1:2000

阳性样品:—

ICC/IF 免疫细胞化学/免疫荧光

物种:Species independent

稀释比:1:100-1:300

阳性样品:HEK-293F细胞, 转染GFP MBP标签构建体的HEK-293F细胞

背景

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

ICC检测MBP Tag蛋白(货号 K5450129)(红色）.
样品: 转染GFP MBP标签构建体的HEK-293F细胞, 4%多聚甲醛 (货号KSG1101) 固定20分钟.
封闭: 3% BSA（货号KSGC305010）的PBS溶液, 室温孵育30分钟.
—抗: 1: 200稀释, 4℃ 孵育过夜.
二抗: Cy3标记山羊抗兔IgG (H+L) (货号KB63909), 1: 300稀释, 室温孵育1小时.

ICC检测MBP Tag蛋白(货号 K5450129)(红色）.
样品: HEK-293F细胞, 4%多聚甲醛 (货号KSG1101) 固定20分钟.
封闭: 3% BSA（货号KSGC305010）的PBS溶液, 室温孵育30分钟.
—抗: 1: 200稀释, 4℃ 孵育过夜.
二抗: Cy3标记山羊抗兔IgG (H+L) (货号KB63909), 1: 300稀释, 室温孵育1小时.