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Anti-Thioredoxin/TRX 兔多克隆抗体
¥1900
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货号: K135871-100
预测分子量: 12 kDa
观测分子量: 12 kDa
别名: Surface associated sulphydryl protein,TRDX,Thioredoxin1,DKFZp686B1993,MGC61975,Trx,ATL-derived factor,ADF,Txn,Txn1,SASP,ATL-derived factor,zgc:92903,TXN,  TRX,TRX1,Thioredoxin,ATL-derived factor,TRDX,Surface-associated sulphydryl protein,Hom s Trx
应用 | 种属
WB H,M,R
规格:
产品信息
蛋白质全称 硫氧还蛋白
别名 Surface associated sulphydryl protein,TRDX,Thioredoxin1,DKFZp686B1993,MGC61975,Trx,ATL-derived factor,ADF,Txn,Txn1,SASP,ATL-derived factor,zgc:92903,TXN,  TRX,TRX1,Thioredoxin,ATL-derived factor,TRDX,Surface-associated sulphydryl protein,Hom s Trx
Uniprot ID P10639
免疫原 KLH偶联的小鼠Thioredoxin合成肽
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 12 kDa / 12 kDa
应用
WB 免疫印迹
物种:人,小鼠,大鼠
稀释比:1:500-1:1000
阳性样品:胰腺
背景
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cystein residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测Thioredoxin/TRX蛋白(货号 K135871).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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