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Anti-Hydrogen Potassium ATPase Beta/ATP4B 兔多克隆抗体
¥1900
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货号: K1335133-100
预测分子量: 34 kDa
观测分子量: 43 kDa
别名: Gastric H(+)/K(+) ATPase subunit beta,Proton pump beta chain,Atp4b,Parietal cell antigen,Gastric hydrogen potassium ATPase,Proton pump
应用 | 种属
WB M,R
规格:
产品信息
蛋白质全称 钾转运ATP酶β亚基
别名 Gastric H(+)/K(+) ATPase subunit beta,Proton pump beta chain,Atp4b,Parietal cell antigen,Gastric hydrogen potassium ATPase,Proton pump
Uniprot ID P50992
免疫原 小鼠Hydrogen Potassium ATPase Beta/ATP4B重组蛋白
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 34 kDa / 43 kDa
应用
WB 免疫印迹
物种:小鼠,大鼠
稀释比:1:500-1:1000
阳性样品:骨骼肌
背景
The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha- subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测Hydrogen Potassium ATPase Beta/ATP4B蛋白(货号 K1335133).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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