Anti-Caldesmon/CDM 兔多克隆抗体-细胞骨架-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-Caldesmon/CDM 兔多克隆抗体

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￥1900

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货号： K135877-100

亚细胞定位：细胞质, 细胞骨架

别名： CDM,L-caldesmon,Cald1,CAD,CALD1,Caldesmon 1,H CAD,LCAD,NAG22,Caldesmon 1 Isoform 1

应用 | 种属

IHC/IF R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	非肌钙调结合蛋白
别名	CDM,L-caldesmon,Cald1,CAD,CALD1,Caldesmon 1,H CAD,LCAD,NAG22,Caldesmon 1 Isoform 1
Uniprot ID	Q62736
免疫原	KLH偶联的人Caldesmon合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞质, 细胞骨架

应用

IHC/IF 免疫组织化学/免疫荧光

物种:大鼠

稀释比:1:1000-1:4000

阳性样品:子宫

背景

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测Caldesmon/CDM蛋白(货号 K135877).
样品: 大鼠子宫, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (KSG1201), 98℃, 20分钟.
—抗: 1: 2500稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.