Anti-GRP94 兔多克隆抗体-细胞器标志物-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-GRP94 兔多克隆抗体

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￥1900

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货号： K1333840-100

预测分子量： 92 kDa

观测分子量： 100 kDa

别名： 94 kDa glucose-regulated protein,GRP-94,ERp99,Heat shock protein 90 kDa beta member 1,Polymorphic tumor rejection antigen 1,Tumor rejection antigen gp96,Hsp90b1,GRP94,Tra-1,Tra1,HSP90B1,ECGP,GP96,GRP94,HEL-S-125m,HEL35,TRA1

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	内质网蛋白
别名	94 kDa glucose-regulated protein,GRP-94,ERp99,Heat shock protein 90 kDa beta member 1,Polymorphic tumor rejection antigen 1,Tumor rejection antigen gp96,Hsp90b1,GRP94,Tra-1,Tra1,HSP90B1,ECGP,GP96,GRP94,HEL-S-125m,HEL35,TRA1
Uniprot ID	P08113
免疫原	小鼠GRP94重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	92 kDa / 100 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:肺

背景

GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测GRP94蛋白(货号 K1333840).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.