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Anti-PRKAR2B 兔多克隆抗体
¥1900
加入对比
货号: K1335160-100
预测分子量: 46 kDa
观测分子量: 46 kDa
别名: Prkar2b,RATDNA,Protein kinase cAMP dependent regulatory type II beta,Pkarb2,H RG363E19.2,RIIbeta
应用 | 种属
WB M,R
规格:
产品信息
蛋白质全称 cAMP依赖性蛋白激酶IIβ调节亚基
别名 Prkar2b,RATDNA,Protein kinase cAMP dependent regulatory type II beta,Pkarb2,H RG363E19.2,RIIbeta
免疫原 小鼠PRKAR2B重组蛋白
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 46 kDa / 46 kDa
应用
WB 免疫印迹
物种:小鼠,大鼠
稀释比:1:500-1:1000
阳性样品:脑
背景
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测PRKAR2B蛋白(货号 K1335160).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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