Anti-PKC gamma 兔多克隆抗体-其它-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-PKC gamma 兔多克隆抗体

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￥1900

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货号： K1346644-100

预测分子量： 78 kDa

观测分子量： 80 kDa

别名： PKC gamma,PKCC,PKCG,PRKCG,Protein kinase C gamma type,protein kinase C,gamma,SCA14

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	蛋白激酶Cγ型
别名	PKC gamma,PKCC,PKCG,PRKCG,Protein kinase C gamma type,protein kinase C,gamma,SCA14
Uniprot ID	P63318
免疫原	KLH偶联的小鼠PKC gamma合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	78 kDa / 80 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:300-1:400

阳性样品:脑

背景

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测PKC gamma蛋白(货号 K1346644).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 300稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.