Anti-ACOT1 兔多克隆抗体-脂代谢-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-ACOT1 兔多克隆抗体

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货号： K1336471-100

预测分子量： 46 kDa

观测分子量： 46 kDa

别名： Acyl-CoA thioesterase 1,CTE-I,Inducible cytosolic acyl-coenzyme A thioester hydrolase,Long chain acyl-CoA hydrolase,Palmitoyl-coenzyme A thioesterase,Acot1,Cte1

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	酰基辅酶A硫酯酶1
别名	Acyl-CoA thioesterase 1,CTE-I,Inducible cytosolic acyl-coenzyme A thioester hydrolase,Long chain acyl-CoA hydrolase,Palmitoyl-coenzyme A thioesterase,Acot1,Cte1
Uniprot ID	O55137
免疫原	小鼠ACOT1重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	46 kDa / 46 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:3000-1:5000

阳性样品:心

背景

Acyl-CoA thioesterases such as ACOT1, hydrolyze acyl-CoAs to the free fatty acid and CoA. ACOTs therefore play key roles in maintaining the intracellular ratio between CoA esters of various lipids and free fatty acids. Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测ACOT1蛋白(货号 K1336471).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 3000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.