Anti-MAP3K12 兔多克隆抗体-其它-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-MAP3K12 兔多克隆抗体

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￥1900

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货号： K1340467-100

亚细胞定位：细胞膜, 细胞质

别名： Dual leucine zipper bearing kinase,Leucine-zipper protein kinase,DLK,ZPK,MAPK-upstream kinase,MUK,Mixed lineage kinase,Map3k12

应用 | 种属

IHC/IF H

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	丝裂原活化蛋白激酶激酶激酶12
别名	Dual leucine zipper bearing kinase,Leucine-zipper protein kinase,DLK,ZPK,MAPK-upstream kinase,MUK,Mixed lineage kinase,Map3k12
Uniprot ID	Q60700
免疫原	小鼠MAP3K12重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞膜, 细胞质

应用

IHC/IF 免疫组织化学/免疫荧光

物种:人

稀释比:1:200-1:400

阳性样品:肾

背景

This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测MAP3K12蛋白(货号 K1340467).
样品: 人肾, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (KSG1201), 98℃, 20分钟.
—抗: 1: 300稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.