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Anti-TrpRS 兔多克隆抗体
¥1900
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货号: K1340875-100
预测分子量: 54 kDa
观测分子量: 54 kDa
别名: GAMMA 2,hWRS,IFI53,IFP53,Interferon induced protein 53,TrpRS,Tryptophanyl-tRNA synthetase,WARS,WARS1,WRS
应用 | 种属
WB H,M,R
规格:
产品信息
蛋白质全称 色氨酰-tRNA合成酶(胞质)
别名 GAMMA 2,hWRS,IFI53,IFP53,Interferon induced protein 53,TrpRS,Tryptophanyl-tRNA synthetase,WARS,WARS1,WRS
Uniprot ID P32921
免疫原 小鼠TrpRS重组蛋白
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 54 kDa / 54 kDa
应用
WB 免疫印迹
物种:人,小鼠,大鼠
稀释比:1:1000-1:2000
阳性样品:睾丸
背景
TrpRS is also named as IFI53, WRS, WARS and belongs to the class-I aminoacyl-tRNA synthetase family. It catalyzes activation of tryptophan by ATP and transfer to tRNATrP, ensuring ranslation of the genetic code for tryptophan. TrpRS is thought to be more closely related to TyrRS than to any other class I enzyme, but the significance of this relationship has been questioned . It can exsit as a dimer. It has 2 isoforms produced by alternative splicing.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测TrpRS蛋白(货号 K1340875).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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