Anti-AMF 兔多克隆抗体-神经发育,营养因子,糖代谢-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-AMF 兔多克隆抗体

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￥1900

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货号： K1341073-100

预测分子量： 63 kDa

观测分子量： 63 kDa

别名： Autocrine motility factor,g6pi,glucose phosphate isomerase,GNPI,GPI,Neuroleukin,NLK,PGI,PHI,Phosphoglucose isomerase,Phosphohexose isomerase,SA 36,Sperm antigen 36,Gpi1

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	葡萄糖-6-磷酸异构酶
别名	Autocrine motility factor,g6pi,glucose phosphate isomerase,GNPI,GPI,Neuroleukin,NLK,PGI,PHI,Phosphoglucose isomerase,Phosphohexose isomerase,SA 36,Sperm antigen 36,Gpi1
Uniprot ID	P06745
免疫原	KLH偶联的小鼠AMF合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	63 kDa / 63 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:1000-1:2000

阳性样品:肺

背景

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis . This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form .

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测AMF蛋白(货号 K1341073).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.