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Anti-RASSF1 兔多克隆抗体
¥1900
加入对比
货号: K1343068-100
预测分子量: 39 kDa
观测分子量: 43 kDa
别名: 123F2,NORE2A,RDA32,REH3P21
应用 | 种属
WB M,R
规格:
产品信息
蛋白质全称 含Ras关联结构域蛋白1
别名 123F2,NORE2A,RDA32,REH3P21
Uniprot ID Q99MK9
免疫原 KLH偶联的小鼠Rassf1合成肽
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 39 kDa / 43 kDa
应用
WB 免疫印迹
物种:小鼠,大鼠
稀释比:1:800-1:1200
阳性样品:肾
背景
Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测RASSF1蛋白(货号 K1343068).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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