Anti-RAB13 兔多克隆抗体-小G蛋白相关-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-RAB13 兔多克隆抗体

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￥1900

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货号： K1346347-100

预测分子量： 23 kDa

观测分子量： 23 kDa

别名： GIG4,Cell growth-inhibiting gene 4 protein,Ras related protein Rab13

应用 | 种属

WB H

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	Ras相关蛋白Rab-13
别名	GIG4,Cell growth-inhibiting gene 4 protein,Ras related protein Rab13
Uniprot ID	Q9DD03
免疫原	小鼠RAB13重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	23 kDa / 23 kDa

应用

WB 免疫印迹

物种:人

稀释比:1:500-1:1000

阳性样品:293细胞

背景

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测RAB13蛋白(货号 K1346347).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.