Anti-Rab1A 兔多克隆抗体-小G蛋白相关-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-Rab1A 兔多克隆抗体

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￥1900

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货号： K1343053-100

预测分子量： 23 kDa

观测分子量： 28 kDa

别名： RAB1,RAB1A,Ras related protein Rab 1A,YPT1,YPT1 related protein

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	Ras相关蛋白Rab-1A
别名	RAB1,RAB1A,Ras related protein Rab 1A,YPT1,YPT1 related protein
Uniprot ID	P62821
免疫原	KLH偶联的小鼠RAB1A合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	23 kDa / 28 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:肝

背景

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes . Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion . RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion (By similarity). Regulates the level of CASR present at the cell membrane (By similarity). Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria (By similarity). Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Rab1A蛋白(货号 K1343053).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.