Anti-RAB27A 兔多克隆抗体-囊泡运输,小G蛋白相关-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-RAB27A 兔多克隆抗体

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￥1900

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货号： K1333318-100

预测分子量： 25 kDa

观测分子量： 28 kDa

别名： GS2,GTP binding protein Ram,HsT18676,Rab 27,RAB27,RAB27A,RAM,Rab27a,Ras-related protein Rab-27A,Ras-related protein Rab27A

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	Ras相关蛋白Rab-27A
别名	GS2,GTP binding protein Ram,HsT18676,Rab 27,RAB27,RAB27A,RAM,Rab27a,Ras-related protein Rab-27A,Ras-related protein Rab27A
Uniprot ID	Q9ERI2
免疫原	KLH偶联的小鼠RAB27A合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	25 kDa / 28 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:肾

背景

Rab27a is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27a is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27a is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测RAB27A蛋白(货号 K1333318).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.