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Anti-Proteasome 19S S4/PSMC1 兔多克隆抗体
¥1900
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货号: K1343281-100
预测分子量: 49 kDa
观测分子量: 60 kDa
别名: P26S4,p56,PSMC1,S4
应用 | 种属
WB H,M,R
规格:
产品信息
蛋白质全称 26S蛋白酶体调节亚基4
别名 P26S4,p56,PSMC1,S4
Uniprot ID P62192
免疫原 小鼠PSMC1重组蛋白
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 49 kDa / 60 kDa
应用
WB 免疫印迹
物种:人,小鼠,大鼠
稀释比:1:2000-1:4000
阳性样品:心, 肾
背景
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC1 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测Proteasome 19S S4/PSMC1蛋白(货号 K1343281).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 2000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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