Anti-TRIM13 兔多克隆抗体-泛素化相关-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-TRIM13 兔多克隆抗体

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货号： K1343884-100

预测分子量： 47 kDa

观测分子量： 47 kDa

别名： B cell chronic lymphocytic leukemia tumor suppressor Leu5; CAR; DLEU5; HGNC:9976; LEU5; Leukemia associated protein 5; Putative tumor suppressor RFP2; Ret finger protein 2; RING finger protein 77; RNF77; TRIM13; Tripartite motif protein 13

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	E3泛素连接酶TRIM13
别名	B cell chronic lymphocytic leukemia tumor suppressor Leu5; CAR; DLEU5; HGNC:9976; LEU5; Leukemia associated protein 5; Putative tumor suppressor RFP2; Ret finger protein 2; RING finger protein 77; RNF77; TRIM13; Tripartite motif protein 13
Uniprot ID	Q9CYB0
免疫原	KLH偶联的小鼠TRIM13合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	47 kDa / 47 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:400-1:800

阳性样品:睾丸

背景

Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor. Plays also a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测TRIM13蛋白(货号 K1343884).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.