Anti-TRIM16 兔多克隆抗体-泛素化相关-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-TRIM16 兔多克隆抗体

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货号： K1335415-100

预测分子量： 63 kDa

观测分子量： 63 kDa

别名： E3 ubiquitin-protein ligase TRIM16,Estrogen-responsive B box protein,Trim16,Ebbp

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	三结构域蛋白16
别名	E3 ubiquitin-protein ligase TRIM16,Estrogen-responsive B box protein,Trim16,Ebbp
Uniprot ID	Q99PP9
免疫原	小鼠TRIM16重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	63 kDa / 63 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:HeLa细胞, A549细胞

背景

The TRIM16 gene was identified as an estrogen and anti-estrogen regulated gene in epithelial cells stably expressing estrogen receptor. TRIM16 contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. The proteins of this family have been reported to be involved in a variety of biological processes including cell growth and differentiation. The TRIM16 gene is expressed in most tissues, and is more highly expressed in the fetus than in the corresponding adult tissues. TRIM16 may play a role in the regulation of keratinocyte differentiation.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测TRIM16蛋白(货号 K1335415).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.