Anti-Proteasome 20S LMP2 兔多克隆抗体-泛素化相关-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-Proteasome 20S LMP2 兔多克隆抗体

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￥1900

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货号： K1340101-100

亚细胞定位：细胞质, 细胞核

别名： 20S proteasome,beta1i,LMP2,Low molecular mass protein 2,Macropain chain 7,Proteasome chain 7,Proteasome subunit beta 1i,Proteasome subunit beta type 9,PSMB6i,PSMB9,RING12

应用 | 种属

IHC/IF M

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	蛋白酶体β9亚基
别名	20S proteasome,beta1i,LMP2,Low molecular mass protein 2,Macropain chain 7,Proteasome chain 7,Proteasome subunit beta 1i,Proteasome subunit beta type 9,PSMB6i,PSMB9,RING12
Uniprot ID	P28076
免疫原	KLH偶联的小鼠Proteasome 20S LMP2合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞质, 细胞核

应用

IHC/IF 免疫组织化学/免疫荧光

物种:小鼠

稀释比:1:300-1:600

阳性样品:心

背景

PSMB9, also named as LMP2, PSMB6i and RING12, belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. High expression of PSMB9 associated with the poor prognosis of patients with NPC.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测Proteasome 20S LMP2蛋白(货号 K1340101).
样品: 小鼠心, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (KSG1201), 98℃, 20分钟.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.