Anti-NAP1L2 兔多克隆抗体-组蛋白相关-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-NAP1L2 兔多克隆抗体

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货号： K1345954-100

亚细胞定位：细胞核

别名： Bpx,Nap1l2

应用 | 种属

IHC/IF H,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	核小体组装蛋白1样2
别名	Bpx,Nap1l2
Uniprot ID	P51860
免疫原	KLH偶联的小鼠NAP1L2合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞核

应用

IHC/IF 免疫组织化学/免疫荧光

物种:人,大鼠

稀释比:1:700-1:2100

阳性样品:肾

背景

Proper nucleosome assembly is critical for compacting DNA into chromatin. In human and mouse there are 5 protein-coding genes which comprise the nucleosome assembly protein (NAP) family.The remaining three family members, NAP1L2, NAP1L3 and NAP1L5 are neuron-specific nucleosome assembly proteins translated from intronless genes which are monoallelically expressed. NAP1L2 (nucleosome assembly protein 1-like 2), also known as BPX (brain specific protein, X-linked), is a 460 amino acid protein containing acidic domains which are thought to mediate histone interactions. NAP1L2 binds to chromatin and interacts with Histones H3 and H4. The function of NAP1L2 is not clearly defined although evidence suggests that NAP1L2 influences histone acetylation and therefore may play a significant role in regulating transcription in developing neurons.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测NAP1L2蛋白(货号 K1345954).
样品: 人肾, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (KSG1203), 98℃, 20分钟.
—抗: 1: 1400稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.