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Anti-MDM2 兔多克隆抗体
¥1900
加入对比
货号: K1346332-100
预测分子量: 54,49 kDa
观测分子量: 40-55 kDa
别名: Double minute 2 protein,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2,hdm2,HDMX,MDM2,p53 binding protein Mdm2
应用 | 种属
WB M,R
规格:
产品信息
蛋白质全称 E3泛素蛋白连接酶Mdm2
别名 Double minute 2 protein,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2,hdm2,HDMX,MDM2,p53 binding protein Mdm2
Uniprot ID P23804
免疫原 KLH偶联的小鼠MDM2合成肽
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 54,49 kDa / 40-55 kDa
应用
WB 免疫印迹
物种:小鼠,大鼠
稀释比:1:300-1:800
阳性样品:脑
背景
MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Over expression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测MDM2蛋白(货号 K1346332).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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