Anti-AKT2 兔多克隆抗体-mTOR通路-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-AKT2 兔多克隆抗体

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￥1900

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货号： K1346410-100

预测分子量： 56 kDa

观测分子量： 60,56 kDa

别名： AKT2,v-akt murine thymoma viral oncogene homolog 2,HIHGHH,PKBB,PKBBETA,PRKBB,RAC-BETA,PKB beta,Protein kinase Akt 2,Protein kinase B beta,RAC PK beta

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	RAC-β丝氨酸/苏氨酸蛋白激酶
别名	AKT2,v-akt murine thymoma viral oncogene homolog 2,HIHGHH,PKBB,PKBBETA,PRKBB,RAC-BETA,PKB beta,Protein kinase Akt 2,Protein kinase B beta,RAC PK beta
Uniprot ID	Q60823
免疫原	KLH偶联的小鼠AKT2合成磷酸肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	56 kDa / 60,56 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:300-1:400

阳性样品:心

背景

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测AKT2蛋白(货号 K1346410).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 300稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.