Anti-PAK4 兔多克隆抗体-其它-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-PAK4 兔多克隆抗体

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￥1900

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货号： K1346473-100

预测分子量： 65 kDa

观测分子量： 68 kDa

别名： KIAA1142,p21 activated kinase 4,PAK 4,PAK4

应用 | 种属

WB H,M

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	丝氨酸/苏氨酸蛋白激酶PAK4
别名	KIAA1142,p21 activated kinase 4,PAK 4,PAK4
Uniprot ID	Q8BTW9
免疫原	KLH偶联的小鼠PAK4合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	65 kDa / 68 kDa

应用

WB 免疫印迹

物种:人,小鼠

稀释比:1:300-1:400

阳性样品:睾丸

背景

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测PAK4蛋白(货号 K1346473).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 300稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.