Anti-alpha 1 Antitrypsin 兔多克隆抗体-细胞骨架-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-alpha 1 Antitrypsin 兔多克隆抗体

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货号： K1346668-100

预测分子量： 47 kDa

观测分子量： 51-55 kDa

别名： AAT,Alpha-1 protease inhibitor 2,Alpha-1-antiproteinase,Serine protease inhibitor 1-2,Serine protease inhibitor A1b,Serpin A1b,Serpina1b,Aat2,Dom2,Spi1-2

应用 | 种属

WB H

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	α1-抗胰蛋白酶
别名	AAT,Alpha-1 protease inhibitor 2,Alpha-1-antiproteinase,Serine protease inhibitor 1-2,Serine protease inhibitor A1b,Serpin A1b,Serpina1b,Aat2,Dom2,Spi1-2
Uniprot ID	P01009
免疫原	KLH偶联的人alpha 1 Antitrypsin合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	47 kDa / 51-55 kDa

应用

WB 免疫印迹

物种:人

稀释比:1:300-1:400

阳性样品:MCF7细胞

背景

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.; [Short peptide from AAT]: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测alpha 1 Antitrypsin蛋白(货号 K1346668).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 300稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.