Recombinant Anti-TNF-alpha antibody (兔单克隆抗体)-细胞因子,重组抗体-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Recombinant Anti-TNF-alpha antibody (兔单克隆抗体)

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￥1900

加入对比

货号： K545564-100

预测分子量： 26 kDa

观测分子量： 25 / 28 kDa

亚细胞定位：细胞膜, 分泌型

别名： TNF,DIF,TNF-alpha,TNFA,TNFSF2,Tumour necrosis factor,Tumour necrosis factor alpha,tumor necrosis factor,TNLG1,TNF-α,TNF a,TNF alpha,NFα,TNF-α,Cachectin,TNFα

应用 | 种属

WB M

IHC/IF M

ICC/IF M

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	肿瘤坏死因子α
别名	TNF,DIF,TNF-alpha,TNFA,TNFSF2,Tumour necrosis factor,Tumour necrosis factor alpha,tumor necrosis factor,TNLG1,TNF-α,TNF a,TNF alpha,NFα,TNF-α,Cachectin,TNFα
Uniprot ID	P06804
免疫原	小鼠TNF-alpha重组蛋白
抗体亚型	IgG, κ
克隆号	SB364
纯化方式	亲和纯化
亚细胞定位	细胞膜, 分泌型
预测分子量./观测分子量.	26 kDa / 25 / 28 kDa

应用

WB 免疫印迹

物种:小鼠

稀释比:1:1000-1:4000

阳性样品:—

IHC/IF 免疫组织化学/免疫荧光

物种:小鼠

稀释比:1:100-1:300

阳性样品:—

ICC/IF 免疫细胞化学/免疫荧光

物种:小鼠

稀释比:1:30-1:100

阳性样品:RAW264.7+LPS细胞

背景

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

ICC检测TNF-alpha蛋白(货号 K545564)(红色）.
样品: RAW264.
7+LPS细胞, 4%多聚甲醛 (货号KSG1101) 固定20分钟.
封闭: 3% BSA（货号KSGC305010）的PBS溶液, 室温孵育30分钟.
—抗: 1: 100稀释, 4℃ 孵育过夜.
二抗: Cy3标记山羊抗兔IgG (H+L) (货号KB63909), 1: 300稀释, 室温孵育1小时.