Recombinant Anti-PD-L1 antibody (小鼠单克隆抗体)-肿瘤标志物,细胞杀伤相关,重组抗体-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Recombinant Anti-PD-L1 antibody (小鼠单克隆抗体)

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￥1900

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货号： K546017-100

预测分子量： 33 kDa

观测分子量： 40-55 kDa

别名： CD274,B7-H,B7H1,PDL-1,PDCD1L1,PDCD1LG1,PDL1,CD274 molecule,Programmed cell death 1 ligand 1

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	程序性细胞死亡蛋白1配体1
别名	CD274,B7-H,B7H1,PDL-1,PDCD1L1,PDCD1LG1,PDL1,CD274 molecule,Programmed cell death 1 ligand 1
Uniprot ID	Q9NZQ7
免疫原	KLH偶联的人PD-L1合成肽
抗体亚型	IgG1, κ
克隆号	SB150
纯化方式	亲和纯化
预测分子量./观测分子量.	33 kDa / 40-55 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:肺

背景

Plays a critical role in induction and maintenance of immune tolerance to self.As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response.Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10).Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis.The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival.The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测PD-L1蛋白(货号 K546017).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗小鼠IgG (H+L) (货号KB69903), 1: 5000稀释, 室温孵育1小时.