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Anti-PD1 兔多克隆抗体
¥1900
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货号: K134014-100
预测分子量: 32 kDa
观测分子量: 40-70 kDa
别名: Protein PD-1,mPD-1,CD279,Pdcd1,Pd1,SLEB2,Systemic lupus erythematosus susceptibility 2,hSLE1,PDCD1,PD-1,PD1,SLEB2,hPD-1,hPD-l,Programmed cell death 1
应用 | 种属
WB M
规格:
产品信息
蛋白质全称 程序性细胞死亡蛋白1
别名 Protein PD-1,mPD-1,CD279,Pdcd1,Pd1,SLEB2,Systemic lupus erythematosus susceptibility 2,hSLE1,PDCD1,PD-1,PD1,SLEB2,hPD-1,hPD-l,Programmed cell death 1
Uniprot ID Q02242
免疫原 小鼠PD1重组蛋白
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 32 kDa / 40-70 kDa
应用
WB 免疫印迹
物种:小鼠
稀释比:1:1000-1:2000
阳性样品:脾, 肺
背景
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) . PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测PD1蛋白(货号 K134014).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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