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Anti-BRSK1 兔多克隆抗体
¥1900
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货号: K1334644-100
预测分子量: 85 kDa
观测分子量: 90 kDa
别名: Brain-specific serine/threonine-protein kinase 1,BR serine/threonine-protein kinase 1,Serine/threonine-protein kinase SAD-B,Brsk1,Gm1100,Sadb
应用 | 种属
WB M
规格:
产品信息
蛋白质全称 丝氨酸/苏氨酸蛋白激酶BRSK1
别名 Brain-specific serine/threonine-protein kinase 1,BR serine/threonine-protein kinase 1,Serine/threonine-protein kinase SAD-B,Brsk1,Gm1100,Sadb
Uniprot ID Q5RJI5
免疫原 小鼠BRSK1重组蛋白
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 85 kDa / 90 kDa
应用
WB 免疫印迹
物种:小鼠
稀释比:1:500-1:1000
阳性样品:脑
背景
BRSK1 was initially identified as a mammalian homolog to the fission yeast S. pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测BRSK1蛋白(货号 K1334644).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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