Anti-ZO1 tight junction protein 兔多克隆抗体-细胞连接-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-ZO1 tight junction protein 兔多克隆抗体

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￥1900

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货号： K133585-100

亚细胞定位：细胞膜, 细胞连接

别名： TJP1,ZO-1,Tight junction protein 1

应用 | 种属

IHC/IF H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	紧密连接蛋白ZO-1
别名	TJP1,ZO-1,Tight junction protein 1
Uniprot ID	P39447
免疫原	KLH偶联的小鼠ZO1 tight junction protein合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞膜, 细胞连接

应用

IHC/IF 免疫组织化学/免疫荧光

物种:人,小鼠,大鼠

稀释比:1:500-1:1200

阳性样品:结肠

背景

Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton.The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation.Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells.Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling.With Tjp2 and TJjp3, participates in the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测ZO1 tight junction protein蛋白(货号 K133585).
样品: 人结肠, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (KSG1201), 98℃, 20分钟.
—抗: 1: 900稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.