Anti-Citrate synthase 兔多克隆抗体-能量代谢-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-Citrate synthase 兔多克隆抗体

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￥1900

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货号： K1335649-100

预测分子量： 52 kDa

观测分子量： 45 kDa

别名： Citrate (Si)-synthase,CS,ACL,ACLY,ATP citrate (pro S ) lyase,ATP citrate lyase,ATP Citrate synthase,ATPCL,Citrate cleavage enzyme,CLATP,EC 2.3.3,EC 2.3.3.1

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	线粒体柠檬酸合酶
别名	Citrate (Si)-synthase,CS,ACL,ACLY,ATP citrate (pro S ) lyase,ATP citrate lyase,ATP Citrate synthase,ATPCL,Citrate cleavage enzyme,CLATP,EC 2.3.3,EC 2.3.3.1
Uniprot ID	Q9CZU6
免疫原	KLH偶联的人Cit大鼠e synthase合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	52 kDa / 45 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:1000-1:1500

阳性样品:心, 脑

背景

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Citrate synthase蛋白(货号 K1335649).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.