Anti-NTH1 兔多克隆抗体-DNA损伤修复-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-NTH1 兔多克隆抗体

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货号： K1338307-100

预测分子量： 34 kDa

观测分子量： 34 kDa

别名： Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase,DNA glycosylase/AP lyase,Nthl1,Nth1,OCTS3

应用 | 种属

WB H

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	内切核酸酶III样1
别名	Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase,DNA glycosylase/AP lyase,Nthl1,Nth1,OCTS3
Uniprot ID	O35980
免疫原	小鼠NTH1重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	34 kDa / 34 kDa

应用

WB 免疫印迹

物种:人

稀释比:1:1000-1:2000

阳性样品:肝

背景

NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测NTH1蛋白(货号 K1338307).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.